Search results for "In vitro rooting"
showing 5 items of 5 documents
In Vitro Rooting of Capparis spinosa L. as Affected by Genotype and by the Proliferation Method Adopted During the Multiplication Phase
2020
The in vitro rooting of three caper (Capparis spinosa L.) selected biotypes, grown in a commercial orchard on the Sicilian island of Salina (38°
Improved micropropagation in Polygala myrtifolia
2004
Stem segments from apical shoot tips of Polygala myrtifolia were used as primary explants to establish in vitro cultures. Axillary shoots produced on noncontaminated explants were excised and recultured in the same medium to increase the stock of shoot cultures. Equal molar concentrations of five cytokinins 2-isopentenyladenine (2iP), kinetin, zeatin, N6-benzyladenine (BA) and adenine were tested for ability to induce axillary shoot development from double node stem segments. The highest rate of axillary shoot proliferation was induced on Murashige and Skoog agar medium supplemented with 1.8 M BA. Seven indole-3-acetic acid (IAA) concentrations (2.8, 5.7, 8.6, 11.4, 14.3, 17.1 M) were t…
Micropropagation of Metrosideros excelsa
2008
Multiple shoots were induced on stem segments of a 8-year-old plant of Metrosideros excelsa Sol ex Gaertn. ‘Parnel’. Axillary shoots produced on uncontaminated explants were excised, segmented and recultured in the same medium to increase the stock of shoot cultures. The Murashige and Skoog (MS) medium, augmented with different concentrations of 2- isopenthenyladenine (2iP) and indole-3-acetic acid (IAA), either singly or in combinations, as potential medium for shoot multiplication by nodal segments was tested. In the following experiment equal molar concentrations of four cytokinins [2iP, kinetin, zeatin and N6-benzyladenine (BA)] in combination with equal molar concentrations of three au…
Micropropagation ofAgeratum houstonianumby nodal segments
2017
Ageratum houstonianum is a bedding and flowering potted plant originated from Central America which is generally propagate by seed. In this report a preliminary in vitro technique for propagation of A. houstonianum was investigated. In vitro germinated seeds were used to establish aseptic shoot cultures of several clones. Seedling stem segments bearing 3-4 nodes were placed on Murashige and Skoog (MS) basal medium plus 20 g L-1 sucrose, 8.0 g L-1 Agar to induce axillary shoot development. Axillary shoots were subcultured into the same medium and nodal segments were sectioned and subcultured to increase the stock of shoot cultures. Shoot cultures of the selected clone AG14 were used to accom…
Micropropagation of Genista aetnensis [(Raf. ex Biv.)DC]
2015
Genista aetnensis [(Raf. ex Biv.)DC] is a large deciduous shrub or small tree native to the Italian islands of Sardinia and Sicily. Being winter hardy and characterized by high plasticity in altitude and ecology, the species is grown in gardens and landscaping, both for flower and for its attractive shape. Genista species are generally propagate by seed or semi hardwood cuttings. In this report an efficient in vitro technique for propagation of G. aetnensis was investigated. Multiple shoots were induced on nodal segments of a mature plant of Genista aetnensis . The Murashige and Skoog medium, augmented with different concentrations of benzyladenine either singly or in combination with indol…